Team:Warsaw/Calendar-Main/14 July 2008

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<p>'''Cloning of protein Z DNA to OmpA constructs'''<br>
<p>'''Cloning of protein Z DNA to OmpA constructs'''<br>
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1. Digestation and dephosphorylatio (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p>
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1. Digestion and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p>
2. Gel eloctrophoresis and gel-out (4300 bp band). <br>
2. Gel eloctrophoresis and gel-out (4300 bp band). <br>
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br>
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br>

Revision as of 13:42, 27 September 2008

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Cloning of protein Z DNA to OmpA constructs
1. Digestion and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.

2. Gel eloctrophoresis and gel-out (4300 bp band).
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.



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