Team:Warsaw/Calendar-Main/21 July 2008

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2. Confirmed transformant colonies inoculated to liquid LB with kanamycin. <br>
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin. <br>
3. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_alpha). <br>  
3. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_alpha). <br>  
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4. Control digestation of isolated plasmids with BamHI and SacI .
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4. Control digestion of isolated plasmids with BamHI and SacI .
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Revision as of 13:44, 27 September 2008

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Cloning of protein A DNA to OmpA constructs
1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.
3. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_alpha).
4. Control digestion of isolated plasmids with BamHI and SacI .



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