Team:Warsaw/Calendar-Main/23 July 2008

From 2008.igem.org

(Difference between revisions)
MKrzyszton (Talk | contribs)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha''' <br> 1. DNA fragment of omega_A isolated from gel on 12 July digested with...)
Newer edit →

Revision as of 15:26, 28 September 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha
1. DNA fragment of omega_A isolated from gel on 12 July digested with SacI and NotI.
2. Clean-up of digested DNA fragment.
3. Digestation (SacI and NotI) and dephosphorylation (CIAP) of pACYC177+OmpA_alpha.
4. Gel electrophoresis and gel-out of 4300 bp band.
5. Ligation of DNA fragments from 2. and 4.



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008"