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Team:Warsaw/Calendar-Main/5 May 2008
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| - | <p>Piotr: Setup of overnight culture <i>E. coli</i> TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria. | + | <p>Piotr: Setup of overnight culture <i>E. coli</i> TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria.<br> |
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'''TOP10 (Invitrogen)'''<br> | '''TOP10 (Invitrogen)'''<br> | ||
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| - | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- <br></p> | + | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- <br> |
| + | </p> | ||
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{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 15:40, 28 September 2008
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Piotr: Setup of overnight culture E. coli TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria.
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