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Team:Warsaw/Calendar-Main/12 May 2008
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<p>1. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</p> | <p>1. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</p> | ||
| - | <p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band.</p> | + | <p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</p> |
| - | <p>3. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI, use of alkaline phosphatase (CIAP).</p> | + | <p>3. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP).</p> |
| - | <p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band.</p> | + | <p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</p> |
<p>5. DNA ligation (pMPM-T5+AID vector)</p> | <p>5. DNA ligation (pMPM-T5+AID vector)</p> | ||
Revision as of 19:12, 28 September 2008
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Michał K.: 1. Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer). 2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). 3. Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). 5. DNA ligation (pMPM-T5+AID vector)  </html>
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