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Team:Warsaw/Calendar-Main/15 May 2008
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<p>Michał K.</p> | <p>Michał K.</p> | ||
| - | <p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID.</p> | + | <p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</p> |
| - | <p>2. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestation</a> of | + | <p>2. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestation</a> of plasmids with HindIII and NcoI (1xTango buffer) - construct control.</p> |
<p>3. Gel electrophoresis - choice of proper clones.</p> | <p>3. Gel electrophoresis - choice of proper clones.</p> | ||
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Michał K. 1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day. 2. Restriction digestation of plasmids with HindIII and NcoI (1xTango buffer) - construct control. 3. Gel electrophoresis - choice of proper clones. |
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