Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
Line 38: | Line 38: | ||
Annealing time: 4 minutes | Annealing time: 4 minutes | ||
- | 20 cycles | + | 20 cycles<br> |
3. PCR - T7 RNA-polimerase for transcription fusion | 3. PCR - T7 RNA-polimerase for transcription fusion | ||
Line 49: | Line 49: | ||
<br> | <br> | ||
</html> | </html> | ||
- | |||
Template DNA: E. coli Rosetta genomic DNA | Template DNA: E. coli Rosetta genomic DNA |
Revision as of 23:08, 28 September 2008
Michał K.: 1. PCR - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Annealing time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
|