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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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| - | 1. PCR - AID for translation fusion | + | 1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">PCR</a> - AID for translation fusion |
Primers: | Primers: | ||
Revision as of 14:30, 29 September 2008
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Michał K.: 1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">PCR</a> - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Elongation time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles
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