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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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| - | 1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols# | + | 1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - AID for translation fusion |
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| - | 2. PCR - T7 RNA-polimerase for translation fusion | + | 2. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polimerase for translation fusion |
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| - | 3. PCR - T7 RNA-polimerase for transcription fusion | + | 3. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA-polimerase for transcription fusion |
Primers: | Primers: | ||
Revision as of 15:23, 29 September 2008
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Michał K.: 1. PCR - AID for translation fusion
Primers:
AIDlNrH
AIDpLinB
Elongation time: 60 sec 20 cycles
Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion
Primers:
T7lRBSHi
T7pXbSal
Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles
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