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Team:Warsaw/Calendar-Main/14 May 2008
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<p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | <p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | ||
| - | 2. Optimalization of conditions for PCR to obtain AID DNA fragment - temperature gradient (from 62°C to 82°C)</p> | + | 2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C). <br> |
| + | Elongation time: 60 sec <br> | ||
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| + | 20 cycles <br></p> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 22:03, 29 September 2008
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Michał K.: 1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
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