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Team:Warsaw/Calendar-Main/14 May 2008
From 2008.igem.org
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<p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | <p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | ||
2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C). <br> | 2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C). <br> | ||
| + | Primers: | ||
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| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a> | ||
| + | <br> | ||
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| + | Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> | ||
| + | </html> <br> | ||
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Elongation time: 60 sec <br> | Elongation time: 60 sec <br> | ||
Revision as of 22:04, 29 September 2008
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Michał K.: 1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
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