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Team:Warsaw/Calendar-Main/14 May 2008
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<p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | <p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br> | ||
| - | 2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C). <br> | + | 2. Optimalization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C). <br> |
Primers: | Primers: | ||
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20 cycles <br> | 20 cycles <br> | ||
| - | 3. Optimalization of conditions for PCR - T7 RNA polymerase for translational fusion; annealing temerature gradient (from 62°C to 82°C). <br> | + | 3. Optimalization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translational fusion; annealing temerature gradient (from 62°C to 82°C). <br> |
Primers: | Primers: | ||
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20 cycles<br> | 20 cycles<br> | ||
| - | 4. Optimalization of conditions for PCR - T7 RNA polymerase for transcription fusion; annealing temerature gradient (from 62°C to 82°C). <br> | + | 4. Optimalization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for transcription fusion; annealing temerature gradient (from 62°C to 82°C). <br> |
Primers: | Primers: | ||
Revision as of 22:17, 29 September 2008
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Michał K.: 1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
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