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Team:Paris/Modeling/f2
From 2008.igem.org
(Difference between revisions)
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|- | |- | ||
|''conversion'' | |''conversion'' | ||
| - | |conversion | + | |conversion ratio between <br> fluorescence and concentration |
|nM.au<sup>-1</sup> | |nM.au<sup>-1</sup> | ||
|(1/79.429) | |(1/79.429) | ||
Revision as of 23:45, 29 September 2008
The experience would give us
Thus, at steady-state and in the exponential phase of growth :
| param | signification | unit | value |
| [expr(pBad)] | expression rate of pBad with RBS E0032 | nM.min-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βbad | production rate of pBad with RBS E0032 not in the system | nM.min-1 | |
| (Kbad/[AraCtot]) | activation constant of pBad not in the system | nM | |
| nbad | complexation order of pBad not in the system | no dimension | |
| Kara | complexation constant Arabinose-AraC not in the system | nM | |
| nara | complexation order Arabinose-AraC not in the system | no dimension |
That will give us directly ƒ2([arab])
