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Team:Warsaw/Calendar-Main/20 May 2008
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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
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Annealing time: 4 minutes <br> | Annealing time: 4 minutes <br> | ||
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35 cycles <br> | 35 cycles <br> | ||
| - | + | 2. Gel electrophoresis. | |
| - | </p> | + | 3. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles. |
| + | <p>Primers: </p> | ||
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| + | <p><html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p> | ||
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| + | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
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| + | Annealing time: 4 minutes <br> | ||
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| + | 4. Gel electrophoresis. | ||
Revision as of 21:26, 30 September 2008
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Michał K: Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
35 cycles 2. Gel electrophoresis. 3. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles. Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
4. Gel electrophoresis.
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