Team:Warsaw/Calendar-Main/21 May 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>Michał K: <br> 1. PCR - transcription fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).</p>...)
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2. Gel electrophoresis. <br>

Revision as of 21:44, 30 September 2008

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Michał K:
1. PCR - transcription fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion


Annealing time: 4 minutes

35 cycles

2. Gel electrophoresis.



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