From 2008.igem.org
(Difference between revisions)
|
|
| Line 4: |
Line 4: |
| | | | |
| | | | |
| - | <p>Michał K: <br>
| |
| - | 1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - transcription fusion: AID + T7 RNA-polymerase - optimalization (temperature gradient 60°C - 80°C).</p>
| |
| | | | |
| - | <p>Primers: </p>
| |
| - |
| |
| - | <p><html>
| |
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and
| |
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p>
| |
| - |
| |
| - |
| |
| - |
| |
| - | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br>
| |
| - |
| |
| - |
| |
| - | Annealing time: 4 minutes <br>
| |
| - |
| |
| - | 35 cycles <br>
| |
| - |
| |
| - | 2. Gel electrophoresis. <br>
| |
| | | | |
| | | | |
Revision as of 22:53, 30 September 2008
"
