Alberta NINT/17 May 2008

From 2008.igem.org

(Difference between revisions)
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               PCR of T/A I oligonucleotide using Term + and Term - primers  
               PCR of T/A I oligonucleotide using Term + and Term - primers  
-
               DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid.
+
               DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
 +
              insertion into plasmid.

Revision as of 20:05, 9 June 2008

lab work (SD, JD):

             PCR of T/A I oligonucleotide using Term + and Term - primers 
             DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
             insertion into plasmid.