Team:Warsaw/Calendar-Main/6 May 2008

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Michał L.: <br>
Michał L.: <br>
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1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta) - template DNA for PCR to obtain T7 polymerase DNA.<br>
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1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA.<br>
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Revision as of 13:14, 1 October 2008

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Piotr:
1. Preparation of electro- and chemocompetent bacteria E. coli TOP10.
2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.
Michał L.:
1. Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA.

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