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Team:Warsaw/Calendar-Main/2 June 2008
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| - | <p>Piotr: <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> | + | <p>Piotr: <br> |
| + | 1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> | ||
| - | <p>Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG</p> | + | <p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p> |
| + | Michał K.: <br> | ||
<h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p> | <h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p> | ||
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Piotr: 2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG. Michał K.:PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymeraseTemplate DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes Number of cycles: 20
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