Team:Warsaw/Calendar-Main/5 July 2008

From 2008.igem.org

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<h3>Preparation of constructs with OmpA protein fusions<br>Michał K.</h3>
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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
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<p><ol>
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1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) <br>  
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<li> Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)</li> <br>  
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2. Control digest of plasmids with NotI and BamHI<br>
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<li> Control digest of plasmids with NotI and BamHI</li>
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3. Gel electrophoresis <br>
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<li> Gel electrophoresis </li>
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</p>
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</ol></p>

Revision as of 16:44, 1 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)

  2. Control digest of plasmids with NotI and BamHI
  3. Gel electrophoresis



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