Team:Warsaw/Calendar-Main/5 July 2008

From 2008.igem.org

(Difference between revisions)
Line 5: Line 5:
<h3>Preparation of constructs with OmpA protein fusions<br>Michał K.</h3>
<h3>Preparation of constructs with OmpA protein fusions<br>Michał K.</h3>
<p><ol>
<p><ol>
-
<li> Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)</li> <br>  
+
<li> Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)</li>  
<li> Control digest of plasmids with NotI and BamHI</li>
<li> Control digest of plasmids with NotI and BamHI</li>
<li> Gel electrophoresis </li>
<li> Gel electrophoresis </li>

Revision as of 16:44, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Preparation of constructs with OmpA protein fusions
Michał K.

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)
  2. Control digest of plasmids with NotI and BamHI
  3. Gel electrophoresis