Team:Warsaw/Calendar-Main/3 June 2008
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<li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper bands. </li> | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper bands. </li> | ||
- | <li> | + | <li>Digest of DNA with HindIII and SalI (2x Tango buffer).</li> |
- | <li> | + | <li>Digest of pMPM-T5 + AID with HindIII and SalI.</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | ||
<li>Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> | <li>Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> |
Revision as of 17:53, 1 October 2008
Preparation of T7 constructs
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