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Team:Warsaw/Calendar-Main/2 June 2008
From 2008.igem.org
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<h3>Piotr:</h3> <br> | <h3>Piotr:</h3> <br> | ||
| - | <p>1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> | + | <p>1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</p> |
<p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG. | <p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG. | ||
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Piotr:1.Transformation of E.coli TOP10 with pZC320. 2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase |
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