Team:Warsaw/Calendar-Main/10 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
<h3>Michał L., Ewa, Marcin</h3>
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<p>Plasmid isolation from night culture of our transformants.<br>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.<br>
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>

Revision as of 18:22, 1 October 2008

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Michał L., Ewa, Marcin

<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
DNA gel electrophoresis of digestion products.
Picking out proper colonies of transformants.

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