Team:Warsaw/Calendar-Main/23 July 2008

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<p>Paweł: ligation results: 15 colonies grown. All of them cultured overnight in LB+amp.</p>

Revision as of 18:23, 1 October 2008

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Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha
1. DNA fragment of omega_A isolated from gel on 12 July digested with SacI and NotI.
2. Clean-up of digested DNA fragment.
3. Digest (SacI and NotI) and dephosphorylation (CIAP) of pACYC177+OmpA_alpha.
4. Gel electrophoresis and gel-out of 4300 bp band.
5. Ligation of DNA fragments from 2. and 4. (1 hr)
6. Transformation of E. coli TOP10 strain with ligation.
7. Transformants plating on LB + kanamycin.

Paweł: ligation results: 15 colonies grown. All of them cultured overnight in LB+amp.

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