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Team:Warsaw/Calendar-Main/25 July 2008
From 2008.igem.org
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2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains). | 2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains). | ||
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| - | + | <p>Gel-out of Z (~200 bp band). Ligation of degested pET15b+OmpA+omega with Z. Ligations transformed into TOP10 strain and plated on LB+ampicillin. </p> | |
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1. Isolation of plasmids from cultures inocluated on previous day. Gel-out of Z (~200 bp band). Ligation of degested pET15b+OmpA+omega with Z. Ligations transformed into TOP10 strain and plated on LB+ampicillin.
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