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Team:Warsaw/Calendar-Main/26 June 2008
From 2008.igem.org
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2. Plating transformants on LB + tetracycline</p> | 2. Plating transformants on LB + tetracycline</p> | ||
| + | <h3> Polymerase Chain Ligation (PCL) </h3> | ||
| + | <table id="result"> | ||
| + | <tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr> | ||
| + | <tr><th>Alpha-A fusion</th><td>Alpha-linker + linker-A</td><td>AlphaL+SacI + AP+NotI </td><td>1500 bp</td></tr> | ||
| + | <tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr> | ||
| + | </table> | ||
| + | <h4>PCL program for fusion A with alpha and omega</h4> | ||
| + | <table id="result"> | ||
| + | <tr><th>Temperature</th><th>Time</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>48°C-62°C</td><td>0:45</td></tr> | ||
| + | <tr><td>72°C</td><td>2:30</td></tr> | ||
| + | <tr><td>72°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
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Revision as of 18:53, 1 October 2008
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Preparation of pMPMT5-AID+AIDT7 construct Polymerase Chain Ligation (PCL)
PCL program for fusion A with alpha and omega
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