Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

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<h3>Piotr:</h3> <br>
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<h3>Piotr:</h3>  
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<p>1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</p>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</li>
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<p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.
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<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>
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<h3><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> for AID transcription fusion with translation fusion AID+T7 RNA-polymerase <br>Michał K.:</h3><p>
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<h3><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> for AID transcription fusion with translation fusion AID+T7 RNA-polymerase <br>Michał K.:</h3>
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Primers:  
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Primers: <br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br></html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>
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Annealing temperature: 55°C<br>
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Elongation temperature: 55°C<br>
Time: 4 minutes<br>
Time: 4 minutes<br>
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Number of cycles: 20
Number of cycles: 20
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Revision as of 18:56, 1 October 2008

Gallery Bricks Notebook Team Project Home


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Piotr:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Michał K.:

Primers:
AIDlNrH and T7pXbSal Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20


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