Team:Warsaw/Calendar-Main/15 July 2008

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<h3>Cloning of protein Z DNA to OmpA constructs<br>
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Michał K.</h3>
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<p><ol><li> 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </li></ol></p>
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<h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Piotr</h3>
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<p><ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pKS+A and pGeneart+Z with SacI and NotI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">pGeneart dephosphorylation with CIAP</a>.</li></ol></p>
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<p>'''Cloning of protein Z DNA to OmpA constructs'''<br>
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<p><ol><li> </li></ol></p>
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1.  4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </p>
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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin



Cloning of protein A DNA to geneart plasmid in place of protein Z DNA
Piotr

  1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pKS+A and pGeneart+Z with SacI and NotI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">pGeneart dephosphorylation with CIAP</a>.

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