Cloning of protein Z DNA to OmpA constructs
Michał K.

1. Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
2. Gel eloctrophoresis and gel-out (4300 bp band).
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.

Cloning of protein A DNA to geneart plasmid in place of protein Z DNA
Antoni

1. Inoculated to liquid LB + ampicylin pGeneart+Z and pKS+A