Team:UNIPV-Pavia/Protocols/PlasmidExtraction

From 2008.igem.org

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*'''QIAGEN Plasmid Mini Kit'''
*'''QIAGEN Plasmid Mini Kit'''
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*You can find the detailed protocol at: [http://www1.qiagen/HB/QIAGENPlasmidPurification_EN http://www1.qiagen/HB/QIAGENPlasmidPurification_EN]
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*
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*Incubate on ice for 30-45 min.
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*We used this kit in the first 2 weeks of our activity, when we didn't have at QIAGEN QIAprep Spin Miniprep Kit our disposal.
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*Heat shock: 42°C for 1 min.
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*Put transformed TOP10 tube on ice and then add 250 µl SOC medium.
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*Incubate 2 hours at 37°C, 220 rpm.
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<h2>QIAGEN QIAprep Spin Miniprep Kit</h2>
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*Centrifuge 10 min, 1200 rpm.
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''(estimated time: 1 hour1)''
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*Remove 150 ul of bacteria-free supernatant.
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*Plate the remaining part of solution (resuspending the bacteria) on a proper agar plate.
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*Incubate overnight at 37°C.
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'''Materials needed:'''
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*'''Overnight mini-culture
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*'''QIAGEN QIAprep Spin- Miniprep Kit'''
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*
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Revision as of 23:51, 9 June 2008

Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook


Plasmid extraction

QIAGEN Plasmid Mini Kit

(estimated time: 3 hours)

Materials needed:

  • Overnight mini-culture
  • QIAGEN Plasmid Mini Kit


  • We used this kit in the first 2 weeks of our activity, when we didn't have at QIAGEN QIAprep Spin Miniprep Kit our disposal.


QIAGEN QIAprep Spin Miniprep Kit

(estimated time: 1 hour1)

Materials needed:

  • Overnight mini-culture
  • QIAGEN QIAprep Spin- Miniprep Kit



Retrieved from "http://2008.igem.org/Team:UNIPV-Pavia/Protocols/PlasmidExtraction"