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Team:NTU-Singapore/Notebook/9 June 2008
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*Choon Kit: | *Choon Kit: | ||
| - | **Amplification of T7 promoter from the Registry(as gel electrophoresis was not successful last week). | + | **Amplification of T7 promoter, 4 empty plasmids ([http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3], [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]) from the Registry (as gel electrophoresis was not successful last week). |
**Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034. | **Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034. | ||
*Chin Chong, Darius: (to be updated) | *Chin Chong, Darius: (to be updated) | ||
Revision as of 02:11, 10 June 2008
Monday 9 June
- Hung,Zhen Fu:
- Prepare plasmid DNA(in liquid form) of :
- GFP producer x 2
- RBS 0032 x 2
- RBS 0034 x 2
- Prepare digestion solutions for 6 above plasmids for later gel electrophoresis.
- Prepare plasmid DNA(in liquid form) of :
- Choon Kit:
- Amplification of T7 promoter, 4 empty plasmids ([http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3], [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]) from the Registry (as gel electrophoresis was not successful last week).
- Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034.
- Chin Chong, Darius: (to be updated)
