From 2008.igem.org
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| - | <h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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| - | <li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
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| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
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| - | <li> Lack confirmed transformant colonies </li>
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Revision as of 23:31, 2 October 2008
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Cloning of protein A DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI .
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł
- Results of ligation: 6 colonies grown.
- Each colony cultured overnight in LB + ampicillin
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