Team:Warsaw/Calendar-Main/21 August 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <br> <h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3> <p><ol> <li> Colony PCR on colonies from pla...)
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Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin
  3. Inoculated to liquid LB with ampicillin pET15b+OmpA-alfa

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