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Team:University of Lethbridge/Notebook/GeneralLabOctober
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====Roxanne==== | ====Roxanne==== | ||
-Inactivated the Enzymes in the morning | -Inactivated the Enzymes in the morning | ||
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-Ran a gel of the tested pLacI, along with the previously cut RFP and TetR in the afternoon on 1% Agarose Gel in TAE. | -Ran a gel of the tested pLacI, along with the previously cut RFP and TetR in the afternoon on 1% Agarose Gel in TAE. | ||
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-the iGEM pLacI had a faint band, RFP looks good (half of it cut), TetR looks like it all cut. Only used 2 uL for each sample, will try running with more. | -the iGEM pLacI had a faint band, RFP looks good (half of it cut), TetR looks like it all cut. Only used 2 uL for each sample, will try running with more. | ||
Revision as of 19:58, 4 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 1, 2008
Roxanne
-Inactivated the Enzymes in the morning
-Ran a gel of the tested pLacI, along with the previously cut RFP and TetR in the afternoon on 1% Agarose Gel in TAE.
-the iGEM pLacI had a faint band, RFP looks good (half of it cut), TetR looks like it all cut. Only used 2 uL for each sample, will try running with more.
October 4, 2008
Roxanne
-Reran the pLacI tests, RFP and TetR on a 1% Agarose Gel in TAE. -Repicked pLacI x2, RFP and TetR colonies into LB+amp media since I've been having trouble with ligations. Brent suggested using lots of DNA in the ligation (<100 ng) to make ure that the ligation does in fact work this time.
