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Team:University of Lethbridge/Notebook/GeneralLabOctober
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-Repicked pLacI x2, RFP and TetR colonies into LB+amp media since I've been having trouble with ligations. Brent suggested using lots of DNA in the ligation (<100 ng) to make ure that the ligation does in fact work this time. | -Repicked pLacI x2, RFP and TetR colonies into LB+amp media since I've been having trouble with ligations. Brent suggested using lots of DNA in the ligation (<100 ng) to make ure that the ligation does in fact work this time. | ||
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Revision as of 20:17, 4 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 1, 2008
Roxanne
-Inactivated the Enzymes in the morning
-Ran a gel of the tested pLacI, along with the previously cut RFP and TetR in the afternoon on 1% Agarose Gel in TAE.
-the iGEM pLacI had a faint band, RFP looks good (half of it cut), TetR looks like it all cut. Only used 2 uL for each sample, will try running with more.
October 4, 2008
Roxanne
-Reran the pLacI tests, RFP and TetR on a 1% Agarose Gel in TAE.
-Repicked pLacI x2, RFP and TetR colonies into LB+amp media since I've been having trouble with ligations. Brent suggested using lots of DNA in the ligation (<100 ng) to make ure that the ligation does in fact work this time.
