Team:Warsaw/Calendar-Main/6 May 2008

From 2008.igem.org

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1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA.<br>
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1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation</a> of genomic DNA (<i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>) - template DNA for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain T7 polymerase DNA.<br>
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Revision as of 20:29, 4 October 2008

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Piotr:

1. Preparation of electro- and chemocompetent bacteria E. coli TOP10.
2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.

Michał L.:

1. Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA.

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