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Team:Warsaw/Calendar-Main/19 May 2008
From 2008.igem.org
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<p>1. Overnight inoculation and induction:</p> | <p>1. Overnight inoculation and induction:</p> | ||
| - | <p>Top10 + 0,5 mM IPTG</p> | + | <p><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> + 0,5 mM IPTG</p> |
<p>pMPM-AID + Glc + 0,01% L-Ara</p> | <p>pMPM-AID + Glc + 0,01% L-Ara</p> | ||
| - | <p>pTrc99a-AID + Glc + 0,5 mM IPTG</p> | + | <p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a> + Glc + 0,5 mM IPTG</p> |
</html> | </html> | ||
Revision as of 20:41, 4 October 2008
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Piotr:1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).
Michał L., Ewa, Marcin:1. Overnight inoculation and induction: Top10 + 0,5 mM IPTG pMPM-AID + Glc + 0,01% L-Ara pTrc99a-AID + Glc + 0,5 mM IPTG
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