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Team:Warsaw/Calendar-Main/26 June 2008
From 2008.igem.org
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<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br> | <p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br> | ||
| - | + | <ol><li>Transformation of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> from previous day </li> | |
| - | + | <li>Plating transformants on LB + tetracycline</li> | |
<h3> Polymerase Chain Ligation (PCL) </h3> | <h3> Polymerase Chain Ligation (PCL) </h3> | ||
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<tr><td>4°C</td><td>infinite</td></tr> | <tr><td>4°C</td><td>infinite</td></tr> | ||
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{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 00:34, 5 October 2008
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'''Preparation of pMPMT5-AID+AIDT7 construct'''
Polymerase Chain Ligation (PCL)
PCL program for fusion A with alpha and omega
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