Team:Warsaw/Calendar-Main/30 June 2008

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<h3>Optimisation of primers from 19.06</h3>
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pDRIVE-TapTag plasmid using
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<p>'''Optimisation of primers from 19.06'''<br>
 
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1. PCR on pDRIVE-TapTag plasmid using
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
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  primers (annealing length 45s)<br>
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  primers (annealing length 45s)</li>
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2. PCR on pUC19 plasmid using  
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pUC19 plasmid using  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
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primers (annealing length 45s)</li>
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primers (annealing length 45s)<br>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pUC19 plasmid using
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3. PCR on pUC19 plasmid using
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
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primers (annealing length 30s)</li>
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<p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p>
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primers (annealing length 30s)<br>
 
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Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p>
 
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Revision as of 01:43, 5 October 2008

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Optimisation of primers from 19.06

  1. PCR on pDRIVE-TapTag plasmid using OmpaL_N and OmpaP_link primers (annealing length 45s)
  2. PCR on pUC19 plasmid using AlphaL_link and AlphaP_XB primers (annealing length 45s)
  3. PCR on pUC19 plasmid using OmegaL_link and OmegaP_EPB primers (annealing length 30s)

    4. Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees

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