Team:Warsaw/Calendar-Main/23 May 2008

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<h4>Michał K.:</h4>
<h4>Michał K.:</h4>
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<li> Inoculation other separate transformant colonies for transcription fusion (liquid LB + tetracycline).</li>
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<li> Inoculation other separate transformant colonies for translation fusion (liquid LB + tetracycline). </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcription fusion from other colonies.</li>
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</ol></p>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion from other colonies.</li>
 
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).</li>
 
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).</li>
 
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<li> Gel electrophoresis (no proper colonies found).</li></html>
 
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Michał K.:

  1. Inoculation other separate transformant colonies for transcription fusion (liquid LB + tetracycline).
  2. Inoculation other separate transformant colonies for translation fusion (liquid LB + tetracycline).

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