Team:Warsaw/Calendar-Main/26 May 2008

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<h4>Michał K.:</h4>
<h4>Michał K.:</h4>
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<li> Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).</li>
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<<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified products from p.1 and p.4.</li>
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<li> Inoculation separate transformant colonies for translation fusion (liquid LB + tetracycline). </li>
 
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligation product.</li>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested products from p.4 and p.5.</li>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligation product.</li>
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<li>  Transformants plating on LB + tetracycline.</li></ol>
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Revision as of 12:17, 5 October 2008

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Michał K.:

    <
  1. Ligation of purified products from p.1 and p.4.
  2. Electroporation of E. coli TOP10 with ligation product.
  3. Ligation of digested products from p.4 and p.5.
  4. Electroporation of E. coli TOP10 with ligation product.
  5. Transformants plating on LB + tetracycline.