"
Team:UNIPV-Pavia/Protocols/PlasmidExtraction
From 2008.igem.org
(Difference between revisions)
| Line 22: | Line 22: | ||
<br> | <br> | ||
'''Materials needed:''' | '''Materials needed:''' | ||
| - | *'''5 ml | + | *'''5 ml or 9 ml (for higher yields) overnight mini-culture |
*'''QIAGEN Plasmid Mini Kit''' | *'''QIAGEN Plasmid Mini Kit''' | ||
<br> | <br> | ||
| Line 33: | Line 33: | ||
<br> | <br> | ||
'''Materials needed:''' | '''Materials needed:''' | ||
| - | *'''5 ml | + | *'''5 ml or 9 ml (for higher yields) overnight mini-culture |
*'''QIAGEN QIAprep Spin Miniprep Kit''' | *'''QIAGEN QIAprep Spin Miniprep Kit''' | ||
<br> | <br> | ||
*We followed standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4] | *We followed standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4] | ||
<br> | <br> | ||
Revision as of 12:31, 10 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Plasmid extraction
We used QIAGEN Plasmid Mini Kit in the first 2 weeks of our activity to perform miniprep, when we didn't have QIAGEN QIAprep Spin Miniprep Kit at our disposal. Then, we always used QIAGEN QIAprep Spin Miniprep Kit for its higher yield.
QIAGEN Plasmid Mini Kit
(estimated time: 3 hours)
Materials needed:
- 5 ml or 9 ml (for higher yields) overnight mini-culture
- QIAGEN Plasmid Mini Kit
- We followed standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=369&tp=4]
QIAGEN QIAprep Spin Miniprep Kit
(estimated time: 1 hour)
Materials needed:
- 5 ml or 9 ml (for higher yields) overnight mini-culture
- QIAGEN QIAprep Spin Miniprep Kit
- We followed standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4]
