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Team:Warsaw/Calendar-Main/21 July 2008
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2. Confirmed transformant colonies inoculated to liquid LB with kanamycin. <br> | 2. Confirmed transformant colonies inoculated to liquid LB with kanamycin. <br> | ||
| - | 3. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_alpha). <br> | + | 3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_alpha). <br> |
| - | 4. Control digest of isolated plasmids with BamHI and SacI . | + | 4. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI . |
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br> | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br> | ||
Paweł</h3> | Paweł</h3> | ||
| - | <p><ol><li>Ligations transformed into TOP10 and plated on LB + ampicillin</li></ol></p> | + | <p><ol><li>Ligations transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB + ampicillin</li></ol></p> |
<p><ol> | <p><ol> | ||
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Revision as of 17:01, 5 October 2008
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Cloning of protein A DNA to OmpA constructs Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
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