Team:Warsaw/Calendar-Main/4 June 2008

(Difference between revisions)

Revision as of 18:19, 5 October 2008


	Attribution analyzer / Interactive list of topics

Digest of DNA with HindIII and SalI (2x Tango buffer).
Digest of pMPM-T5 + AID with HindIII and SalI.
Clean-up of reaction products.
Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).
Transformation of E.coli TOP10 with ligation product.
Plating transformants on LB+tetracycline.

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 {{WarNotebook}}
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+<html><ol>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of DNA with HindIII and SalI (2x Tango buffer).</li>
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 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li>
-<li>Plating transformants on LB+tetracycline.</li></ol>
+<li>Plating transformants on LB+tetracycline.</li></ol></html>