Team:Warsaw/Calendar-Main/5 September 2008

(Difference between revisions)

Revision as of 22:58, 5 October 2008


	Attribution analyzer / Interactive list of topics

Bacteria centrifuged and sonicated (according to protocol).
Sonicate centrifuged.
In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20
In case of A-alpha: pellet discarded, supernatant kept for further purification steps

@@ Line 2: / Line 2: @@
 <!-- do not edit above me! -->
+<html>
+<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>Piotr</h3>
+<p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
+<li>Sonicate centrifuged. </li>
+<li>In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20</li>
+<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p>
+<br>
+<h3>Protein A mutagenesis<br>Paweł</h3>
+<p><ol><li>Treatment of mutageneses as on 2nd September. </li></ol></p>
-Oczyszczanie białek: A-alfa, Z-alfa i Z-omega
+</html>
-Piotr
-Zwirowanie bakterii z 1l, sonikacja ich (protokół)
-zwirowanie sonikatu
-w przypadku z-alfa i z-omega odżucenie supernatantu, zawieszenie osadu w wodzi i ponowne zwirowanie i odżucenie wody i zawieszenie w wodzie
-w przypadku a-alfa zatrzymanie supernatantu odżucenie osadu
 <!-- do not remove this! -->
 {{WarNotebookEnd}}