Team:UNIPV-Pavia/Protocols/GelExtraction
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Revision as of 13:29, 10 June 2008
Home | The Team | The Project | Biological Safety | Parts Submitted to the Registry |
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Dry Lab | Wet Lab | Modeling | Protocols | Activity Notebook |
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
DNA gel extraction
(Estimated time: 1 hour)
Materials needed:
- A gel in which the bands of interest can be seen
- Scalpel and tweezers to cut gel
- Roche Agarose Gel DNA Extraction Kit
- Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol