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Team:ESBS-Strasbourg/6 October 2008
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(New page: < back == DryLab == === Modeling === == WetLab == ''Katja:'' * Verification PCR for Leu2 and Ura3 parts -> negative * Ligation and Transformation of:...)
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(New page: < back == DryLab == === Modeling === == WetLab == ''Katja:'' * Verification PCR for Leu2 and Ura3 parts -> negative * Ligation and Transformation of:...)
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Revision as of 17:28, 6 October 2008
Contents |
DryLab
Modeling
WetLab
Katja:
- Verification PCR for Leu2 and Ura3 parts -> negative
- Ligation and Transformation of:
- Ura3 (FI with Pst1 site) + pSB1A2(FV)
- Leu2 (BI with EcoRI site) + pSB1A2 (BV)
- Gal1 (BV)+ CFP_NLS_adhterm (BI)
- Gal1 (BV)+ CFP_cln2_adhterm (BI)
- Gal1 (BV)+ CFP_hsl1_adhterm (BI)
- Gal1 (BV)+ mCherry_L_lexA_L_VP16_L (BI) (Ligation by Sandra)
- Gal1 (BV)+ lexA_L_VP16_L_mCherry_L (BI) (Ligation by Sandra)
- Preparation of LB-media (2x150ml, 2x100ml)
- Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm
