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Team:ESBS-Strasbourg/6 October 2008
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(Difference between revisions)
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== WetLab == | == WetLab == | ||
| - | ''Katja:'' | + | '''Katja:''' |
* Verification PCR for Leu2 and Ura3 parts -> negative | * Verification PCR for Leu2 and Ura3 parts -> negative | ||
* Ligation and Transformation of: | * Ligation and Transformation of: | ||
| - | ** Ura3 (FI with Pst1 site) + pSB1A2(FV) | + | ** Ura3 (FI with Pst1 site) + pSB1A2 (FV) |
** Leu2 (BI with EcoRI site) + pSB1A2 (BV) | ** Leu2 (BI with EcoRI site) + pSB1A2 (BV) | ||
** Gal1 (BV)+ CFP_NLS_adhterm (BI) | ** Gal1 (BV)+ CFP_NLS_adhterm (BI) | ||
| Line 18: | Line 18: | ||
* Preparation of LB-media (2x150ml, 2x100ml) | * Preparation of LB-media (2x150ml, 2x100ml) | ||
* Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm | * Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm | ||
| + | |||
| + | ''goals for tomorrow:'' | ||
| + | * Verification of: | ||
| + | ** Ura3 | ||
| + | ** Leu2 | ||
| + | ** Gal1_CFP_NLS_adhterm | ||
| + | ** Gal1_CFP_cln2_adhterm | ||
| + | ** Gal1_CFP_hsl1_adhterm | ||
| + | ** Gal1_mCherry_L_lexA_L_VP16_L | ||
| + | ** Gal1_lexA_L_VP16_L_mCherry_L | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Revision as of 17:31, 6 October 2008
Contents |
DryLab
Modeling
WetLab
Katja:
- Verification PCR for Leu2 and Ura3 parts -> negative
- Ligation and Transformation of:
- Ura3 (FI with Pst1 site) + pSB1A2 (FV)
- Leu2 (BI with EcoRI site) + pSB1A2 (BV)
- Gal1 (BV)+ CFP_NLS_adhterm (BI)
- Gal1 (BV)+ CFP_cln2_adhterm (BI)
- Gal1 (BV)+ CFP_hsl1_adhterm (BI)
- Gal1 (BV)+ mCherry_L_lexA_L_VP16_L (BI) (Ligation by Sandra)
- Gal1 (BV)+ lexA_L_VP16_L_mCherry_L (BI) (Ligation by Sandra)
- Preparation of LB-media (2x150ml, 2x100ml)
- Start of ON of lexA_L, mCherry_L_VP16_L, cin8_adhterm
goals for tomorrow:
- Verification of:
- Ura3
- Leu2
- Gal1_CFP_NLS_adhterm
- Gal1_CFP_cln2_adhterm
- Gal1_CFP_hsl1_adhterm
- Gal1_mCherry_L_lexA_L_VP16_L
- Gal1_lexA_L_VP16_L_mCherry_L
