"
Team:University of Lethbridge/Notebook/Project4October
From 2008.igem.org
Munima.alam (Talk | contribs) m (link to go back to the main notebook) |
Munima.alam (Talk | contribs) (Oct 6 and 7) |
||
| Line 1: | Line 1: | ||
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | [[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | ||
| + | |||
| + | ===October 6, 2008=== | ||
| + | ====Jaden==== | ||
| + | Objective: Grow up cells containing the xylE gene from the glycerol stocks and plates (Selina had transformed with DH5a) in LB + Amp liquid media. | ||
| + | The gene was sent to us in pVL1392 (modified pUC8 for viral expression, with Amp resistance). | ||
| + | |||
| + | Subcultured four tubes: Two from glycerol stocks and two from colonies on a plate. | ||
| + | |||
| + | |||
| + | ===October 7, 2008=== | ||
| + | ====Christa, Munima==== | ||
| + | Objective: Plasmid prep the xylE gene that Jaden subcultured yesterday. | ||
| + | |||
| + | Christa: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the Eppendorf FastPlasmid Mini Prep Kit. Stored 50 uL aliquots in the -20 C iGEM freezer labeled "xylE from glycerol stock" and "xylE from plate", and have a big blue "P" or "G" on the top, respectively. | ||
| + | |||
| + | Munima: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the QiaPrep Mini Kit. Plasmids are stored in 50 uL aliquots in the -20 C iGEM freezer. They are labeled "xylE plasmid; plate 2; Oct. 7/08" and "xylE plasmid; glycerol 2; Oct. 7/08". | ||
Revision as of 03:31, 8 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 6, 2008
Jaden
Objective: Grow up cells containing the xylE gene from the glycerol stocks and plates (Selina had transformed with DH5a) in LB + Amp liquid media. The gene was sent to us in pVL1392 (modified pUC8 for viral expression, with Amp resistance).
Subcultured four tubes: Two from glycerol stocks and two from colonies on a plate.
October 7, 2008
Christa, Munima
Objective: Plasmid prep the xylE gene that Jaden subcultured yesterday.
Christa: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the Eppendorf FastPlasmid Mini Prep Kit. Stored 50 uL aliquots in the -20 C iGEM freezer labeled "xylE from glycerol stock" and "xylE from plate", and have a big blue "P" or "G" on the top, respectively.
Munima: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the QiaPrep Mini Kit. Plasmids are stored in 50 uL aliquots in the -20 C iGEM freezer. They are labeled "xylE plasmid; plate 2; Oct. 7/08" and "xylE plasmid; glycerol 2; Oct. 7/08".
